Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF) in Skin Rejuvenation After 40

By Barbara Dobosz

Advanced Aesthetic Practitioner & Educator

Barbara Dobosz is a highly accomplished cosmetologist and aesthetic medicine specialist with 17 years of clinical experience. Founder of two clinics in Poland, she specialises in advanced anti-aging therapies and mature skin stimulation.

Her philosophy centres on preserving a youthful appearance through subtle enhancement, ensuring full respect for a patient’s natural facial features without the risk of overcorrection. In clinical practice, she utilises a sophisticated combination of platelet-rich plasma, fibrin therapies, biostimulators, volumetric techniques, and HIFU technologies, always prioritising safety, precision, and natural-looking results.

• Website: https://artskinakademia.pl/
• Instagram: https://www.instagram.com/barbara.dobosz.artskin/

Biological Restoration of Fat Pads, Oxygenation, and Deep Regeneration Unmatched by Any Other Treatment**

After the age of 40, the skin undergoes several structural changes that significantly impact facial appearance. It is not only about wrinkles or elasticity, but also the deep layers of the face—fat pads, vascular density, and the thickness of the dermis. The most noticeable transformations occur in areas such as the temples, mid-cheek region, and lateral chin. These changes lead to hollows, shadows, and loss of youthful contour, making the face appear tired and older.

This is where Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF) become invaluable. These autologous regenerative therapies not only rejuvenate the skin but also rebuild tissues, enhance oxygenation, restore vascular networks, and bring back the youthful architecture of the face. They activate biological mechanisms that no other aesthetic procedure can replicate.

Why Are PRP and PRF So Effective After 40?

With age, the following changes occur:

• 30–40% loss of facial fat pads,
• decreased skin density and elasticity,
• slower fibroblast activity and reduced collagen production,
• reduced vascularization and oxygen supply,
• visible sagging and volume depletion.

PRP and PRF address every layer of these processes.

1. The Only Treatment That Creates a NEW Network of Blood Vessels

Growth factors—especially VEGF—trigger neovascularization, meaning the formation of new microvessels.

This results in:

• improved oxygenation,
• enhanced nutrient delivery,
• faster cellular regeneration,
• restored radiance and unified tone,
• increased density and firmness.

No laser, no filler, and no skin booster is capable of creating new capillary networks. This effect is exclusive to PRP and PRF.

2. Restoration of Facial Fat Pads for a Naturally Younger Contour

After 40, facial fat pads diminish, particularly in:

• temples,
• mid-cheek region,
• lateral chin and jawline,
• under-eye area (tear trough).

PRF, thanks to its gel-like consistency, functions as a natural, biological volumizer, allowing:

• filling of hollow areas,
• lifting of sagging tissues,
• structural regeneration,
• restoring the appearance of youthful fat pads.

The effect is extremely natural—never artificial or heavy.

Why Blood Quality = Treatment Quality?

Importance of a 2-Week Pre-Treatment Diet

The treatment is only as effective as the biological material it is made from—the patient’s blood. For this reason, overall health and dietary habits directly influence the outcome.

For at least 14 days before the procedure, it is recommended to:

1. Hydrate Intensively
   Low hydration = low plasma yield = weaker separation.
2. Avoid Alcohol for 5–7 Days
   Alcohol reduces platelet quality and impairs tissue repair.
3. Increase Antioxidants
   Green leafy vegetables, berries, and omega-3 fatty acids reduce oxidative stress.
4. Reduce Sugar and Processed Foods
   Sugar causes collagen glycation, slowing regeneration.
5. Supplement When Necessary
   Vitamin C, zinc, and iron support platelet function.

Preparing the body properly dramatically increases the effectiveness of PRP and PRF.

Centrifugation Parameters

The Key to an Effective Procedure

Modern regenerative medicine emphasizes minimizing platelet trauma to maximize biological activity.

PRP (Platelet-Rich Plasma)

✔ 5 minutes
✔ 1500 rpm

These parameters allow optimal platelet concentration without damaging their structure.

PRF (Platelet-Rich Fibrin)

✔ 3 minutes
✔ 700 rpm

This low-speed technique preserves:

• live leukocytes,
• mesenchymal stem cells,
• a natural fibrin matrix,
• slow-release growth factors for up to 14 days.

Low-speed centrifugation is now the gold standard for achieving superior regenerative effects.

The Importance of Medical-Grade Tubes

Not all tubes are suitable for PRP/PRF. For maximum platelet survival and biological activity, tubes must be:

Sterile, medical-grade, made of appropriate material (glass or medical polymer)

This ensures:

• chemical stability,
• no contamination,
• prevention of platelet damage,
• extended viability of growth factors,
• better clinical outcomes.

Correct tubes + proper centrifugation + immediate application = maximum therapeutic effectiveness.

Injection Techniques

Precise Modeling Without Fillers

Depending on the area and goal, two advanced techniques are used:

1. Linear (Retrograde) Technique

Ideal for:

• nasolabial folds,
• marionette lines,
• jawline contour,
• overall skin thickening.

The product is applied along the tissue plane during cannula or needle withdrawal.

2. Point-Deposit (Depot) Technique

Used for:

• tear trough,
• cheek hollows,
• temples,
• localized volume loss.

It allows precise, targeted placement of PRP/PRF for natural volumization.

Recommended Treatment Series

How Many Procedures After 40 and After 50?

To achieve full regeneration, fat pad restoration, vascular renewal, and improved density, PRP and PRF must be performed in a series, since biological processes require repeated stimulation.

Optimal Interval Between Treatments

Every 3–4 weeks, ensuring continuous activation of growth factors.

How Many Treatments After Age 40?

The skin still has relatively active fibroblasts and good vascular potential.

Recommended: 2 treatments of combined PRP + PRF, every 3–4 weeks.

How Many Treatments After Age 50?

Regeneration slows, and volume loss becomes more pronounced.

Recommended: 3 treatments of combined PRP + PRF, every 3–4 weeks.

Why Combine PRP and PRF?

Because they complement each other:

• PRP provides strong stimulation and improved skin quality,
• PRF restores lost volume and slowly rebuilds tissue.

Together, they create a biological facelift effect, naturally and safely.

Summary – The Unique Power of PRP and PRF After 40

PRP and PRF are the only treatments that:

✓ create new blood vessels (oxygenation and deep nourishment),
✓ thicken and densify the skin,
✓ stimulate fibroblasts and collagen remodeling,
✓ restore lost fat pads (temples, cheeks, jawline),
✓ improve the tear trough, folds, and hollows naturally,
✓ regenerate tissues at a cellular level,
✓ offer long-lasting results (especially PRF),
✓ are 100% natural and safe.

All of this is achieved using the patient’s own blood—without fillers, without foreign substances, without the risk of complications.

Biological rejuvenation is the foundation of modern aesthetic medicine, especially for women after 40 who need not only smoothing of the skin but true structural restoration and regeneration.